Edited by Sarah A. Woodson, Johns Hopkins University, Baltimore, MD, and accepted by Editorial Board Member Michael F. Summers March 16, 2021 (received for review November 10, 2020)Transcription–translation coupling is critical for fast and efficient gene expression in many bacteria, yet how coupling between RNA polymerase (RNAP) and ribosome is established and maintained is poorly understood. Combining biochemical and single-molecule fluorescence approaches, we here uncover the roles of nascent mRNA structure and transcription factors in coupling. We find that the inherently paused RNAP...…Edited by Sarah A. Woodson, Johns Hopkins University, Baltimore, MD, and accepted by Editorial Board Member Michael F. Summers March 16, 2021 (received for review November 10, 2020)Transcription–translation coupling is critical for fast and efficient gene expression in many bacteria, yet how coupling between RNA polymerase (RNAP) and ribosome is established and maintained is poorly understood. Combining biochemical and single-molecule fluorescence approaches, we here uncover the roles of nascent mRNA structure and transcription factors in coupling. We find that the inherently paused RNAP...WW…

Patients with COVID-19 are at high risk for thrombotic arterial and venous occlusions. Lung histopathology often reveals fibrin-based occlusions in the small blood vessels of patients who succumb to the disease. Antiphospholipid syndrome is an acquired and potentially life-threatening thrombophilia in which patients develop pathogenic autoantibodies targeting phospholipids and phospholipid-binding proteins (aPL antibodies). Case series have recently detected aPL antibodies in patients with COVID-19. Here, we measured eight types of aPL antibodies in serum samples from 172 patients...…Patients with COVID-19 are at high risk for thrombotic arterial and venous occlusions. Lung histopathology often reveals fibrin-based occlusions in the small blood vessels of patients who succumb to the disease. Antiphospholipid syndrome is an acquired and potentially life-threatening thrombophilia in which patients develop pathogenic autoantibodies targeting phospholipids and phospholipid-binding proteins (aPL antibodies). Case series have recently detected aPL antibodies in patients with COVID-19. Here, we measured eight types of aPL antibodies in serum samples from 172 patients...WW…

You are currently viewing the abstract.AAAS login provides access to Science for AAAS members, and access to other journals in the Science family to users who have purchased individual subscriptions.Log in via OpenAthens.Log in with your institution via Shibboleth.Download and print this article for your personal scholarly, research, and educational use.Buy a single issue of Science for just $15 USD.As humans age, we prioritize established positive friendships over the new, but risky, socializing we do when we are young. It has been hypothesized that this shift may come as our own sense of...…You are currently viewing the abstract.AAAS login provides access to Science for AAAS members, and access to other journals in the Science family to users who have purchased individual subscriptions.Log in via OpenAthens.Log in with your institution via Shibboleth.Download and print this article for your personal scholarly, research, and educational use.Buy a single issue of Science for just $15 USD.As humans age, we prioritize established positive friendships over the new, but risky, socializing we do when we are young. It has been hypothesized that this shift may come as our own sense of...WW…

Heat shock factor–1 (HSF-1) is a master regulator of stress responses across taxa. Overexpression of HSF-1 or genetic ablation of its conserved negative regulator, heat shock factor binding protein 1 (HSB-1), results in robust life-span extension in Caenorhabditis elegans. Here, we found that increased HSF-1 activity elevates histone H4 levels in somatic tissues during development, while knockdown of H4 completely suppresses HSF-1–mediated longevity. Moreover, overexpression of H4 is sufficient to extend life span. Ablation of HSB-1 induces an H4-dependent increase in micrococcal nuclease...…Heat shock factor–1 (HSF-1) is a master regulator of stress responses across taxa. Overexpression of HSF-1 or genetic ablation of its conserved negative regulator, heat shock factor binding protein 1 (HSB-1), results in robust life-span extension in Caenorhabditis elegans. Here, we found that increased HSF-1 activity elevates histone H4 levels in somatic tissues during development, while knockdown of H4 completely suppresses HSF-1–mediated longevity. Moreover, overexpression of H4 is sufficient to extend life span. Ablation of HSB-1 induces an H4-dependent increase in micrococcal nuclease...WW…