Edited by Thomas J. Silhavy, Princeton University, Princeton, NJ, and approved February 25, 2021 (received for review September 1, 2020)The outer membrane of gram-negative bacteria is a major deterrent for antibiotic entry, making it difficult to treat these infections. It is composed of both LPS and glycerophospholipids, where the synchronized synthesis between these two components is essential for the outer membrane’s unique permeability barrier. In this report, we identify a previously unidentified association between LPS and glycerophospholipids, where the presence of the complete...…Edited by Thomas J. Silhavy, Princeton University, Princeton, NJ, and approved February 25, 2021 (received for review September 1, 2020)The outer membrane of gram-negative bacteria is a major deterrent for antibiotic entry, making it difficult to treat these infections. It is composed of both LPS and glycerophospholipids, where the synchronized synthesis between these two components is essential for the outer membrane’s unique permeability barrier. In this report, we identify a previously unidentified association between LPS and glycerophospholipids, where the presence of the complete...WW…